Coumarin fluorescence dequenching assay.
figure
posted on 2012-10-19, 01:37 authored by Xiaohui Wang, Zeno Fiorini, Christina Smith, Yingning Zhang, Jing Li, Linda R. Watkins, Hang Yin(a), Schematic representation of coumarin fluorescence dequenching assay. Coumarin labeled TMD-5 peptide forms trimers in the micelle and coumarin fluorescence is self-quenched. A small molecule that disrupts the TMD-5 oligomerization will suppress the quenching of coumarin fluorescence and a fluorescence increase will be observed. (b), Pentamidine disrupts LMP-1 TMD-5 trimers in the micelle. Pentamidine (64 µM) was added into 100 nM of coumarin labeled TMD-5 solution (50 mM HEPES, pH = 7.4) with 150 µM C14 betaine. Excitation, 360 nm; emission, 430 nm. *p<0.05 by student t-test versus DMSO control.
