Construction of chimeric protein YopE_1–138/VEGFR2_352–411 and its secretion efficacy.

A Schematic representation of the translational fusion of YopE_1–138 and VEGFR2_352–411 encoded by plasmid pHR584. B Western blot analysis of hybrid protein YopE_1–138/VEGFR2_352–411 showing its stable production and efficient secretion into the Salmonella culture supernatant. SB824 (pHR241) expressing YopE_1–138-p60 served as a positive and plasmid-less SB824 as a negative control.