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Construction of cDNA library from Upland cotton anthers.

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posted on 2012-11-14, 00:47 authored by Jianhui Ma, Hengling Wei, Meizhen Song, Chaoyou Pang, Ji Liu, Long Wang, Jinfa Zhang, Shuli Fan, Shuxun Yu

Flower buds (A) and the corresponding anther samples (B) were divided into 12 development stages. Anthers in stage 1 were at MEP (C) and anthers in stage 2 were at TTP (D). (E) Average insert size of the cDNA library was determined by PCR analysis of randomly selected clones. DL 2000 plus markers (lane M) were used for size determination.

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