Confirmation of stable PLZF overexpression in HEK293T cells.

(A) HEK293T cells were stably transfected with an expression vector encoding PLZF (PLZF/pCEP4) or an insertless control plasmid (pCEP4). Transcript levels were quantified by real-time PCR in technical triplicates. The numbers 1 to 3 indicate total RNA preparations used for microarray analyses. Standard deviations are given with respect to all columns. (B) Total protein was extracted from HEK293T cells as used in (A) and subjected to Western blotting using an anti-PLZF antibody (sc-28319, Santa Cruz Biotechnology).