Comparison of reactivity for Leishmania antibody detection by rKLO8 ELISA, DAT and rK39 strip test.
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posted on 2013-07-18, 01:50 authored by Elfadil Abass, Nadine Bollig, Katharina Reinhard, Bärbel Camara, Durria Mansour, Alexander Visekruna, Michael Lohoff, Ulrich SteinhoffODs for 106 VL sera diluted 1∶800 were measured in the rKLO8 ELISA and compared with DAT antibody titres or strip test results. (A) Sera were divided into 4 groups based on DAT titres; negative, 1∶±1600; weak, 1∶3200–1∶6400; moderate, 1∶12800–1∶25600; strong, 1∶≥51200. (B) Mean ODs for VL sera with negative or positive DAT titres were compared. Results are expressed as mean ± SD. P value (Mann-Whitney U-test). (C) OD values for VL sera with negative or positive strip test results were compared. (D) Mean ELISA OD values for VL sera with positive or negative strip test results. Dots represent values for individual sera and horizontal lines represent cut-off values.
