Comparison between ESR1 and ER protein on Yale breast cancer cohorts.

The natural log of the nuclear ER AQUA score determined by QIF using SP1 is shown on the y-axis and the AQUA score for ESR1 determined by qISH is on x-axis for YTMA 128, n = 167 with scores for both SP1 and ESR1 (A) and YTMA 130 1993–2005 Subset, n = 195 with scores for both SP1 and ESR1 (B). The dotted line represents the threshold for ER protein positivity. (C) Representative images from 4 cases on YTMA 128 for cytokeratin (green), DAPI (blue), and ESR1 or ER SP1 (red).