Comparison and assignation of growth cone collapse state.

A) Distributions of Z-scores of the 10 Conographer-derived parameters in extended (blue) and collapsed (red) GCs. B) Concordance between human observers (black dotted line) and K-means clustering based upon PCA (red), and incorporation of multiple variables ranked by either their individual concordance (grey) or the difference between Z-score means (blue). C) Collapsed (red) and extended (blue) GCs, as determined by a human, plotted upon the first 2 principal components (PCs) derived from Z-scores. D) Collapsed (red) and extended (blue) GCs as determined by K-means clustering, plotted on the same axis as C; white-centred points indicate the GCs furthest from the centre of the cluster to which they do not belong. E) 5 extended and collapsed growth cones, as determined by K-means clustering. F) Growth cones misidentified by K-means clustering as either collapsed or extended.