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Combinations of either NVP-BKM120 and rapamycin or LY294002 and rapamycin in H2170 (left) and SW900 (right) lung cancer cell lines.

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posted on 15.02.2012, 00:45 by Christopher R. Zito, Lucia B. Jilaveanu, Valsamo Anagnostou, David Rimm, Gerold Bepler, Sauveur-Michel Maira, Wolfgang Hackl, Robert Camp, Harriet M. Kluger, Herta H. Chao

Cells were treated with descending nanomolar concentrations of the PI3K inhibitor NVP-BKM120 or LY294002, alone or combined with 1 nM, 10 nM or 100 nM rapamycin for 72 hours and viability was measured with the Cell Titer Glo assay. The bars show viability as a percentage of viable cells relative to untreated cells. Three separate experiments were performed and each condition was measured in three replicate wells.

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