Combinations of either NVP-BKM120 and rapamycin or LY294002 and rapamycin in H2170 (left) and SW900 (right) lung cancer cell lines.

Cells were treated with descending nanomolar concentrations of the PI3K inhibitor NVP-BKM120 or LY294002, alone or combined with 1 nM, 10 nM or 100 nM rapamycin for 72 hours and viability was measured with the Cell Titer Glo assay. The bars show viability as a percentage of viable cells relative to untreated cells. Three separate experiments were performed and each condition was measured in three replicate wells.