Colocalization of TC10 with Rab11 on vesicles.

N1E-115 cells expressing mCherry-Rab11 and mTFP-TC10 were serum-starved for 6 h and imaged every 10 sec. (A) A representative image of the subcellular distribution of TC10 and Rab11 in a growth cone. A merged image (bottom-right) shows remarkable colocalization between TC10 and Rab11 on vesicles. A bar, 5 μm. (B) A scatter plot depicts the percentage of Rab11-containing vesicles that also have TC10. A blue bar indicates the median value (n = 35). (C) mTFP (TC10), mCherry (Rab11), and merged images corresponding to the boxed region in (A) at the indicated time points are shown. Blue arrowheads mark the co-migration of TC10 and Rab11 on vesicles. (D) Representative images of vesicular localization of TC10 and Rab11 in a neurite shaft at the indicated time points. The movement of one vesicle containing both TC10 and Rab11 is traced with a dotted line. A bar, 5 μm.