Colocalization of 5HTR_B- and PTTH-ir in the adult BR-SOG of

A. pernyi. BmPTTH-ir/ApPTTH-ir in the DL region of protocerebrum of female adult less than one day old was co-localized with Ap5HTR_B-ir (red filled circles). EH-ir in the adult brain of A. pernyi and its colocalization with Ap5HTR_B-ir (blue filled circles) and unique distribution (open circles) of EH-ir in other regions of the brain. (A) The location of detected cells. Lower-case letters correspond to the regions shown in the photographs (e.g., b to B). (B) Two pairs BmEH-ir cells in the DC region. (C, G) 5HTR_B-ir in the DL region. (D) BmPTTH-ir in the DL region. (E) Merged image of BmPTTH-ir and 5HTR_B-ir in the DL region. (F) ApPTTH-ir in the DL region. (H) Merged image of ApPTTH-ir and 5HTR_B-ir in the DL region. (I) A 5HTR_B-ir cell in the SOG. (J) One BmEH-ir cell in the SOG. (K) Merged image of BmEH-ir and 5HTR_B-ir in the SOG region. Scale bar = 100 µm.