Cloning and protein expression of full-length genes and full-length proteins.
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posted on 2016-02-09, 09:52 authored by Daniel O. Connor, Jonas Zantow, Michael Hust, Frank F. Bier, Markus von Nickisch-RosenegkA 1.5% agarose gel: M Hyperladder I (bioline, Bio-33025-26), 1 NGO0170, 2 NGO0326, 3 NGO0451, 4 NGO0564 (+ control), 5 NGO0642, 6 NGO0777, 7 NGO0916, 8 NGO1043, 9 NGO1500 (- control), 10 NGO1634, 11 NGO1656, 12 NGO1657, 13 NGO1796, 14 NGO1852. B SDS-PAGE (15%) of 0.5 μg each of produced proteins: M Spectra Multicolor Low Range Protein Ladder (Thermo Scientific, 26628), 1 NGO0326, 2 NGO0777, 3 NGO1043, 4 NGO 1657, 5 NGO1852. C SDS-PAGE (10%) of 0.5 μg each of produced proteins: M PageRuler Plus Prestained Protein Ladder (Thermo Scientific, 26619), 1 NGO0170, 2 NGO0451, 3 NGO0564 (+ control), 4 NGO642, 5 NGO1500 (- control), 6 NGO1634, 7 NGO1656, 8 NGO1796.
