Cleavage site mapping of selected miRNAs using modified RLM-RACE.
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posted on 2014-03-26, 03:57 authored by Ankur R. Bhardwaj, Gopal Joshi, Ritu Pandey, Bharti Kukreja, Shailendra Goel, Arun Jagannath, Amar Kumar, Surekha Katiyar-Agarwal, Manu AgarwalTargets of the B. juncea miR156 (Bju-miR156), miR160 (Bju-miR160) and miR164 (Bju-miR164) were predicted by psRNA target finder. Primers were designed on the basis of the predicted sequences (TC2101178, TC165518 and TC211305). Cleavage products of three miRNA targets were amplified from RACE library, cloned in pGEMT vector and sequenced. The binding region between the miRNAs and the targets is shown. The arrows indicate cleavage site of transcript and the number of positive clones out of the total clones sequenced is indicated in parentheses above the arrow.
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biotechnologyPlant biotechnologyPlant genomicscell biologyCell processesCellular stress responsesMolecular cell biologyComputational biologygenome analysisGene ontologiesTranscriptome analysisComparative genomicsgeneticsepigeneticsRNA interferencegenomicsFunctional genomicsgene expressionorganismsplantsseedlingsPlant sciencemirnasmodified
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