Classification of alternative splicing events.

(A) Number of splicing events and genes detected for 5 classes of alternative splicing events: cassette exons, alternative 5′ splice sites, alternative 3′ splice sites, mutually exclusive exons and retained introns. (B) Percentage of each class of alternative splicing event shown in piechart. (C) Frame preservation in constitutively and alternatively spliced cassette exons; phase 2 and phase 1 exons are out-of-frame; phase 0 exons are in-frame. (D) RT-PCR validation of strain-specific alternative splicing events in non-irradiated (−) and irradiated (+) sexual and asexual animals. A cassette exon (CUFF.250649) is shown in top gel, while a retained intron (CUFF.62385) is shown in the bottom gel.