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Chromatin immunoprecipitation (ChIP) assay in bone marrow derived macrophages stimulated with LPS and mIFNγ.

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posted on 2013-02-20, 22:37 authored by Tadayuki Akagi, Nils H. Thoennissen, Ann George, Gay Crooks, Jee Hoon Song, Ryoko Okamoto, Daniel Nowak, Adrian F. Gombart, H. Phillip Koeffler

Bone marrow derived macrophages stimulated with LPS and mIFNγ were subjected to ChIP assay by using anti-C/EBPβ, anti-C/EBPε or normal IgG antibodies. C/EBP-binding region of the Marco (A) and Clec4e (B) genes were amplified by specific primers.

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