Characterization of the polarization profile of monocytes, MΦ^GM-CSF (MΦ-1 model) and MΦ^M-CSF (MΦ-2 model).

Monocytes were cultured for 5 days in complete medium or 50% L1236 or HDLM2 SN. MΦ^GM-CSF and MΦ^M-CSF were treated for 24 h with medium or 50% L1236 or HDLM2 SN. The expression of different MΦ lineage markers was determined, including CD68, CD163, CD206 and HLA-DR. Mean fluorescence intensity (MFI) is indicated on the bar charts. Paired student's t-tests or Wilcoxon signed-rank tests were conducted with *P<0.05, **P<0.01 and ***P<0.001.