Characterization of PGC-1α-FAT-KO mice.

(A) Mass of major white and brown adipose tissues of 16-week old Wt (white bars) and PGC-1α-FAT-KO (black bars) mice fed a regular diet (Gon., visceral gonadal; Ing., subcutaneous inguinal; Ren., visceral retroperitoneal; BAT, interescapular brown adipose tissue) (n = 9–10 animals/group). (B) Weight of tissues of Wt and PGC-1α-FAT-KO mice (Gastroc., gastrocnemius muscle). (C) mRNA expression of mitochondrial genes of the OxPhos system or genes involved in fatty acid oxidation was determined by real-time quantitative PCR. (D) Mitochondrial mass in WAT was estimated by measuring mtDNA content by quantitative real-time PCR. Results are expressed as mean ± SEM. (n = 5 animals/group).