Characterization of C3201-HSA fusions.
(A) Fusions of C3201 and HSA induce luciferase activity on an FGF21-responsive engineered reporter cell line following four hour stimulation of serum-starved cells. Potency is influenced by the orientation of the fusion. Luciferase activity is measured in relative luminescence units (RLU). Data are represented as mean +/− SEM (n = 2). (B) Fusions of C3201 and HSA induce ERK phosphorylation in cultured primary human adipocytes. Potency is influenced by the orientation of the fusion. Data are expressed as % ERK protein that is phosphorylated, as measured by MSD.