Cerebellar lobules of tamoxifen-injected Nse-CreER^T2;mTmG mice labeled for different neuronal marker proteins.

A-E: mGFP-immunoreactivity is shown in green (mGFP), immunoreactivity for different cell type specific markers is shown in the red channel (labeled with blue fluorescence). Arrowheads point to the fissure between two lobules. A: NeuN (in the cerebellum a GC-specific marker), B: GFAP (produced by cells of glial lineage), C: Calbindin (CB, produced by Purkinje cells), D: MAP2 (located in neuronal dendrites), and E: Parvalbumin (PV, expressed by Purkinje cells and ML interneurons). NeuN was mainly found in the GCL and cells were co-immunoreactive for GFP. GFAP-labeled structures showed no GFP-signal, nor did Purkinje cells or the interneurons of the ML. MAP2-positive structures were densely immunostained in the GCL, representing the dendrites of GCs. Scale bar  = 50 µm.