Cell viability and DNA damage were determined after methyl methanesulfonate (MMS) treatment.
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posted on 2016-01-28, 12:37 authored by Xiangjing Gao, Guanglin Zhang, Shigang Shan, Yunlong Shang, Linfeng Chi, Hongjuan Li, Yifei Cao, Xinqiang Zhu, Meibian Zhang, Jun Yang(A) HeLa cells were treated with the indicated concentrations of MMS for 12, 24, 36, and 48 h. Cell viability was measured by MTT assay. (B) HeLa cells were treated with different concentrations of MMS for 12 h, then detected the level of DNA damage by immunofluorescence (a) and Western blot (b). Scale bar, 50 μm. The results are shown as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, as compared with the solvent control group.
