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Cell replication assay.

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posted on 2014-10-23, 03:27 authored by Weiwei Li, Gang Wang, Wulong Liang, Kai Kang, Kangkang Guo, Yanming Zhang

Cells were cultivated for 12 h, 24 h, 48 h and 72 h in 96-well plate before adding 100 µl MTT (5 mg/ml) per well. Absorbance value for each well was collected at 570 nm using Multiskan FC Microplate Photometer. Y-axis denotes relative absorbance value at 570 nm while the absorbance value at 12 h was set as 1.

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