Cd-elevated [Ca2+]i induces ROS in neuronal cells.

Indicated cells were exposed to 0–20 µM Cd for 24 h after pretreatment with/without indicated concentrations of BAPTA/AM (A) or EGTA (B) for 30 min, followed by ROS detection, as described in Materials and Methods. Results are presented as mean ± SE; n = 6. aP<0.05, bP<0.01, difference vs. control group; cP<0.01, difference vs. 10 µM Cd group; dP<0.01, difference vs. 20 µM Cd group.