Caspase-11 mediates inflammasome activation in response to a functional Yersinia type III secretion system.
BMDMs from B6, Casp1−/−Casp11−/−, Casp1−/−, or Casp11−/− mice were primed with 0.05 µg/mL LPS for 4 hours and infected with type III secretion system-deficient Y. pseudotuberculosis (ΔyopB Yp), effectorless Y. pseudotuberculosis ΔHOJMEK (Δ6 Yp), or PBS (mock infection) or treated with 2.5 mm ATP for 4 hours. (A) Levels of IL-1α and IL-1β in the supernatants were measured by ELISA. (B) Cell death (% cytotoxicity) was measured by lactate dehydrogenase (LDH) release relative to Triton X-100-lysed cells. Graphs show the mean ± SEM of triplicate wells. Data are representative of two independent experiments. *** is p<0.001 and ** is p<0.01 by two-way ANOVA with Bonferroni post-test. NS is not significant.