Calbindin staining in cerebellum of late stage 22L-animals highlights progressive dendritic pathology but limited presynaptic degeneration in VGLUT1 reactive parallel fibre synapses.

Calbindin immunoreactivity (green) illustrates the severe loss of Purkinje cells dendrites in the molecular layer of the cerebellum and a reduction in Purkinje cell number (nuclear stain, blue) at LS p.i. in 22L-animals. VGLUT1-positive (red) parallel fibers and synaptic terminals are uniformly distributed throughout the molecular layer and do not exhibit any detectable alterations as revealed from images at increasing magnifications from A to C. Scale bars: 385 µM (A); 110 µm (B); 58 µm (C).