CFTR channel in MNS and MHS DCT cells.

A) Representative traces of the rat 8 pS chloride channel recorded at positive or negative potentials in DCT cells isolated from 11 MHS and 16 MNS rats. 0 indicates the closed level. B) Mean current-voltage (I-V) curve of CFTR in MNS (n = 3) and MHS DCT cells (n = 8). C) Open probability (Po) curve of CFTR in MNS (n = 3) and MHS DCT cells (n = 8). D) Representative trace showing inhibition by CFTR-(inh)-172. E) Histograms representing the channel open probability (Po) before or after 2 µmol/L CFTR-(inh)-172 exposure (n = 4). **P<0.01. F) Channel open probability (Po) after 10 µmol/L forskolin and 2 µmol/L CFTR-(inh)-172 exposure (n = 4). G) Confocal images showing CFTR expression in MNS and MHS DCT cells. CFTR is located both in the plasma membrane and in intracellular vesicles. Primary antibody: anti-CFTR; secondary antibody: Cy2 anti-rabbit. Blue: nuclei DAPI staining. Scale bar is 20 µm.