CD44 is a positive fibroblast marker and a negative PSC marker.
figure
posted on 2014-01-09, 03:20 authored by Rene H. Quintanilla Jr, Joanna S. T. Asprer, Candida Vaz, Vivek Tanavde, Uma Lakshmipathy(A) CD44 immunostaining of (i) MEFs, (ii) BJ fibroblasts, (iii) feeder-free H9 ESCs, (iv) feeder-free iPSCs, (v) H9 ESCs on MEF feeders, and (vi) iPSCs on MEF feeders. The merged images shown consist of phase contrast and CD44 signal (green) (Scale bar: 200 µm). (B) Flow cytometry histograms of CD44-Alexa Fluor® 488 signal intensity in stained samples (solid black line) and unstained samples (dotted gray line) of (i) MEFs, (ii) BJ fibroblasts, (iii) feeder-free H9 ESCs, (iv) feeder-free iPSCs, (v) H9 ESCs on MEF feeders, and (vi) iPSCs on MEF feeders. (FF = feeder-free).
