CD3⁺ T cell proliferation monitored using CFSE labeling.

CFSE labeled splenocytes (2×10⁶ cells/well) were treated with or without 2 µmol/L of sodium selenite in the absence or presence of anti-CD3, ConA or PHA, and proliferation was analyzed at 48 h of culture. Cells were stained with anti-CD3-PEcy5. CD3⁺ T cells from the entire well were analyzed for proliferation by flow cytometry. The percentage of proliferating cells for each culture is indicated. A representative experiment from two separate experiments is shown.