Brainstem cutting planes and transverse sections.

(A) Brainstem sagittal view demonstrating cutting planes. The blocks containing the rostral interstitial nucleus of the medial longitudinal fascicle (RIMLF), the oculomotor nucleus (nIII), the paramedian pontine reticular formation (PPRF) including the excitatory (EBN) and inhibitory burst neurons (IBN), the nucleus raphe interpositus (RIP) containing omnipause neurons (OPN), and the abducens nucleus (nVI), were cut in series of 10μm and 5μm thick sections. (B) Caudal view of the block containing the RIMLF. (C) Caudal view of the block containing the PPRF and OPN region. Scale bar B,C = 1cm. INC, interstitial nucleus of Cajal; IO, inferior olive; MB, mammillary body; LGN, lateral geniculate nucleus; MCP, medial cerebellar peduncle; MGN, medial geniculate nucleus; MT, mammillothalamic tract; nIV, trochlear nucleus; NVI, abducens nerve; PC, posterior commissure; RN, red nucleus; TR, tractus retroflexus; PC, posterior commissure; PUL, pulvinar; SC, superior colliculus; SCP, superior cerebellar peduncle; SN, substantia nigra.