Bnl is expressed in the somatic muscle and ectoderm.

In bnl-lacZ expressing embryos, (A–E), Bnl (A–E, green) is expressed in the dorsal-most row of muscle cells (A, arrows) and in single cells in the dorsal (B, arrows) and lateral muscles (C, arrows) labeled with β3t (A–C, red). Bnl (D, green) is not expressed in the dorsal epidermis labeled with E-cad (D, arrows, red). Bnl (E, arrows, green) is expressed in the epidermal cells of the segmental grooves labeled with E-cad (E, red). All embryos shown are at stage 14. Embryos in A–C were stained for β3t (red) and β-gal (green) whereas embryos in D and E were stained for E-cad (red) and β-gal. Scale bar in A represents 5 µm.