Blood circulation kinetics of HPMA copolymer shielded AdHexCys.

To analyze whether shielding with HPMA copolymers would alter the blood circulation kinetics of AdHexCys we injected BALB/c mice with HPMA copolymer-shielded vector particles. 2, 4, 6, 10 and 20 min after vector delivery 20 of blood were taken from the tail vein with a glass capillary by scoring. Total DNA was extracted and Ad genome content was analyzed by QPCR. HPMA copolymers # 268, # 269, # 279 and # 283 not tested. Abbreviations: HexCys: unshielded AdHexCys, the “+ Polymer-number” indicates a shielding of AdHexCys with the respective HPMA copolymer, neut.: neutral (uncharged), pos.: positively charged, rev.: bioresponsive (PySS-) shielding, rel. AUC: relative area under the curve.