Binding of recombinant PACC to the <i>gsn</i> promoter.

<p>(A) Upper panel, schematic representation of the <i>pacC</i> probe and the specific competitor oligo <i>pacC</i>. Lower panels, gel shift analysis using increasing amounts of recombinant PACC in the presence of specific competitors and polyclonal anti-PACC antibody. Lanes 1, 5 and 9, <i>pacC</i> probe, no protein added. Lanes 2, 6 and 10, gel shift analysis using 1.0, 2.0 and 5.0 µg of recombinant PACC. Lanes 3 and 7, gel shift analysis in the presence of the 146 bp specific competitor. Lanes 4 and 8, gel shift analysis using the specific competitor oligo <i>pacC</i>. Lanes 11 to 13, supershift assay using 5.0, 10.0 and 20.0 µL of anti-PACC antibody (1∶500). (B) Upper panel, schematic representation of the mutated m<i>pacC</i> probe. Lower panel, gel shift with wild-type and mutated probes. Analysis using different concentrations of recombinant PACC in the presence and in the absence of competitors. Lane 1 and 3, <i>pacC</i> and m<i>pacC</i> probes, respectively, no protein added. Lanes O, gel origin; SC, specific competitor; FP, free probe.</p>