Binding of His₆-EF-Tu to immobilized PGM.

(A) PGM-coated wells were incubated with 0.5–20 µg His₆-EF-Tu at pH 5.0 or 7.2. (B) Effects of treatment with sulfatase or sialidase on the adhesion of His₆-EF-Tu to PGM. Detection of bound His₆-EF-Tu in the presence of anti-His antibodies. Asterisks indicate that His₆-EF-Tu binding to glycosidase-digested PGM was significantly different (*p<0.05) from binding to untreated control PGM, as determined by Student’s t-test. For all studies, error bars indicate standard deviations (n = 3).