Binding of F4ab fimbriae to reference glycosphingolipids.

Chemical detection by anisaldehyde (A), and autoradiograms obtained by binding of ¹²⁵I-labeled F4ab fimbriae (B). The lanes were: Lane 1, sulfatide (SO₃-3Galß1Cer) with d18:1-24:1 ceramide, 4 µg; Lane 2, sulfatide with d18:1-h16:0 ceramide, 4 µg; Lane 3, sulfatide with t18:0-h24:0 ceramide, 4 µg; Lane 4, sulf-lactosylceramide (SO₃-3Galß4Glcß1Cer) with t18:0-h16:0 ceramide, 4 µg; Lane 5, reference sulf-gangliotetraosylceramide (SO₃-3Galß3GalNAcß4Galß4Glcß1Cer), 4 µg. The glycosphingolipids visualized with anisaldehyde in (A) are marked with Roman numbers, and the corresponding glycosphingolipid structures are given below the chromatograms.