Bacterial Abundance in the Mesenteric Lymph Nodes (MLN) of Mice.
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posted on 2015-07-08, 03:43 authored by Zachary M. Earley, Suhail Akhtar, Stefan J. Green, Ankur Naqib, Omair Khan, Abigail R. Cannon, Adam M. Hammer, Niya L. Morris, Xiaoling Li, Joshua M. Eberhardt, Richard L Gamelli, Richard H. Kennedy, Mashkoor A. ChoudhryMLN were aseptically removed from sham, burn day 1, and burn day 3 animals and Enterobacteriaceae were quantified by qPCR and standardized by total bacterial 16s rRNA gene abundance, (A). Data were expressed as mean ± SEM of 5–11 animals per group. Enterobacteriaceae abundance from sham and burn day 3 animals were all not detectable (ND). In addition, MLN homogenates were plated on Tryptic Soy Agar with 5% sheep blood and MacConkey Agar plates and cultured aerobically for 24 hours with 5% CO2. (B) is a representative image of plates produced from one animal.
