BIRC6 knockdown sensitized CRC cells to cisplatin (CDDP) or 5-fluorouracil (5-FU)-induced apoptosis.

(A) Representative images of cell apoptosis assessed by Annexin V/PI staining in the control (CS) and BIRC6 knockdown cells treated with CDDP (40 μM for SW480 or 100 μM for DLD-1) for 24 h or not. (B) Representative images of cell apoptosis for the control and BIRC6 knockdown cells treated with 5-FU (75 μg/mL for SW480 or 50 μg/mL for DLD-1) for 48 h or not. (C) Quantification of the apoptosis rate. Data present Mean ± SEM from three independent experiments. **, P < 0.01 vs. control.