Figure_1.tif (2.35 MB)
BCR-ABL downregulates PTEN expression.
figure
posted on 2014-10-24, 15:19 authored by Cristina Panuzzo, Sabrina Crivellaro, Giovanna Carrà, Angelo Guerrasio, Giuseppe Saglio, Alessandro MorottiA) Left panel: evaluation of PTEN levels of expression in BCR-ABL-pcDNA3.1 transfected NIH3T3. Right panel: quantification of PTEN levels. B) PTEN mRNA levels in BCR-ABL transfected NIH3T3 cells. C) Left panel: PTEN levels by western immunoblot in primary CD34 positive cells obtained from the bone marrow of normal individuals and Chronic Myeloid Leukemia patients. Right panel: quantification of PTEN levels. D) Stably expressing pBabe-BCR-ABL infected NIH3T3 cells were treated with MG132 and UO126 for 8 hours. Control: parental NIH3T3 expressing pBABE-empty vector.
