Attachment dependent activation of the JNK pathway.

S2R+ cells transiently transfected with dJun-FRET biosensor (A) or the controls mCFP alone (B) and mCFP-dJun (C) were plated on different surfaces and the mCFP donor FLs were collected 48 hours post transfection. dJun-FRET biosensor activity (A) was highest on cells plated on glass (black), and much less pronounced on collagen-coated glass (red), and on Con-A-coated glass (blue). FL of control biosensors was not affected by the substrate (panels B, C).