Assessment of signal consistence of IFP1.4 labeled CPC.

(A) Correlation between CPC numbers and near infrared signals, IFP1.4-labeled CPC at 2.5×10⁵, 1.25×10⁵, 6.25×10⁴, 3.125×10⁴, and 1.57×10⁴ were seeded into 24-well plate, and incubated with 25 µM biliverdin overnight, and then subjected to scanning on the Odyssey Infrared imager; (B) Assessment of near infrared signal intensity showed a robust linear correlation (R² = 0.9927) between the cell number and near infrared signal; (C–D) Comparison of near infrared signal from CPC ^IFP1.4 at P6, P7, P8 and P9 with/without Biliverdin treatment.