Aspirin-induced ROS production.

Intracellular production of ROS was measured using DCFDA and fluorescence was measured by flow cytometry using Cell Quest software as described in the Materials and Methods. ROS was measured in GSH depleted HepG2 cells treated with different concentrations of aspirin and also after NAC treatment (Figure 3i). Result expressed is a typical representation of three determinations. % change in ROS production from a typical histogram is presented in Figure 3ii. Asterisks indicate significant difference (P≤0.05) from control values, # indicate significant difference (P≤0.05) from 5 µmol/ml ASA treated group and δ indicate significant difference (P≤0.05) as compared to 10 µmol/ml ASA treated group.