Apico-basolateral polarity of SOLD1 secretion from BT-1 cells.
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posted on 2013-02-21, 05:55 authored by Koichi Ushizawa, Toru Takahashi, Misa Hosoe, Keiichiro Kizaki, Kazuyoshi Hashizume(A) Western blot analysis of BT-1 conditioned medium and BT-1 cell lysate. (B) Results of the SOLD1 binding assay. BT-1 cells were cultured and removed on COL1-A coated plates. The plates were washed ten times with TBST. SOLD1 binding to COL1-A coated plates was measured by monitoring absorbance at 405 nm in each of four wells per sample. The means±SEM of triplicate measurements are shown. Values with asterisks are significantly different (P<0.05).
