Antinociceptive effect of GW9508 and DHA on CFA-induced mechanical allodynia and thermal hyperalgesia.

GW9508 (1.0 µg) and DHA (50 µg) i.c.v. administered in CFA-injected mice, whereas GW1100 (10 µg) was administered via the i.c.v. route 10 min before GW9508 or DHA injection; CFA-induced mechanical allodynia and thermal hyperalgesia are examined at day 1 (A, B) and 7 (C, D) post-injection; data, mean ± S.E.M.; Saline (n = 6), CFA (Day 1) (n = 6), CFA (Day 1)+GW9508 (1 µg) (n = 6), CFA (Day 1)+GW9508 (10 µg) (n = 6), CFA (Day 1)+GW9508 (25 µg) (n = 6), CFA (Day 1)+GW9508 (25 µg) (n = 6), CFA (Day7) (n = 6), CFA (Day 7)+GW9508 (1 µg) +GW1100 (1 µg) (n = 6), CFA (Day 7)+GW9508 (1 µg) +GW1100 (10 µg) (n = 6), CFA (Day 7)+DHA (50 µg) (n = 6), CFA (Day 7)+DHA (50 µg)+ GW1100 (10 µg) (n = 6); ^$p<0.05, compared with saline; *p<0.05, compared with CFA; ^#p<0.05, compared with CFA+GW9508 (Scheffe's test).