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Analyzing maturation times (MT).

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posted on 2013-10-14, 03:20 authored by Elke Hebisch, Johannes Knebel, Janek Landsberg, Erwin Frey, Madeleine Leisner

A) Typical data set of fluorescence development of the S strain expressing GFP in the absence (grey triangles) and presence (black circles) of 200 µg/ml CAP. An arrow indicates the addition of CAP after 120 min. B) Typical data set of fluorescence development of the S strain expressing GFP in the presence of 200 µg/ml CAP. The arrow indicates the addition of CAP. Fluorescence development is fitted with an exponential fit, yielding τGFP at normalized 63% FI. The maturation time then corresponds to the inverse of the characteristic time constant τGFP of the exponential fit. C) Typical data set of fluorescence development of the S strain expressing mCh in the presence of 200 µg/ml CAP. The arrow indicates the addition of CAP. Fluorescence development is fitted with a sigmoidal fit, yielding τmCh at normalized 63% FI. The maturation time then corresponds to the inverse of the characteristic time constant τmCh of the sigmoidal fit.

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