Analytical gel filtration chromatography of Thermus thermophilus MDH (TtMDH).

TtMDH (0.1 ml with 8.8 mg/ml) was subjected to Superdex 200 HR and the elution profile was compared with high-molecular-weight (HMW) standard proteins (thyroglobulin, 669 kDa; ferritin, 440 kDa; aldolase, 158 kDa; conalbumin, 75 kDa; and ovalbumin, 43 kDa, obtained from GE Healthcare). TtMDH showed a peak with estimated molecular mass 61 kDa. Inset, elution volumes of peaks of HMW standards (•) and peak TtMDH (▴) in the logarithmic scale.