Figure_3.tif (915.59 kB)
Analysis of signal transduction in muscle lysates from 11-, 29- and 54-week female DU6P and Fzt:DU mice.
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posted on 2013-02-20, 03:04 authored by Mandy Sawitzky, Anja Zeissler, Martina Langhammer, Maximilian Bielohuby, Peggy Stock, Harald M. Hammon, Solvig Görs, Cornelia C. Metges, Barbara J. M. Stoehr, Martin Bidlingmaier, Carolin Fromm-Dornieden, Bernhard G. Baumgartner, Bruno Christ, Bertram Brenig, Gerhard Binder, Friedrich Metzger, Ulla Renne, Andreas HoeflichThe Western blot inserts show phosphorylated (Ser-473) and total expression of AKT from one representative experiment, whereby all samples were studied on the identical membrane. Each Western blots were performed three times. Thus a total of 9 different animals was included in the bar chart for each timepoint. Specific activation was calculated from the ratios of phosphorylated versus total AKT (n = 9 per age group). Coomassie blue staining of the membranes used for Western immuno detection was used as loading control. The error bars represent SEM.
