Figure_7.tif (1000.13 kB)
Analysis of protein metabolism in female DU6P and Fzt:DU mice.
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posted on 2013-02-20, 03:08 authored by Mandy Sawitzky, Anja Zeissler, Martina Langhammer, Maximilian Bielohuby, Peggy Stock, Harald M. Hammon, Solvig Görs, Cornelia C. Metges, Barbara J. M. Stoehr, Martin Bidlingmaier, Carolin Fromm-Dornieden, Bernhard G. Baumgartner, Bruno Christ, Bertram Brenig, Gerhard Binder, Friedrich Metzger, Ulla Renne, Andreas HoeflichA: Western blot of muscle lysates in 11-, 29- and 54-week DU6P and Fzt:DU mice identified different proteins tagged with Ubiquitin for protein degradation (n = 5 per age group). Intensities of the complete lanes were calculated and normalized for the Coomassie blue signals present in the identical complete lane on the membranes used for Western immuno detection. The insert provides a representative experiment, whereby all samples were studied on the identical membrane. Serum levels of 1/3-methyl-histidine (B) and ornithine (C) were analyzed by quantitative HPLC as described in Materials and Methods. The error bars represent SEM.
