Analysis of miR-886-3p effect on 3′-UTR of CDC6.

A) pEZX-WT-UTR vector with both Renilla luciferase (hRLuc) was used as an internal control and firefly luciferase (hLuc) was upstream of the 3′-UTR construct. B) Putative binding site of miR-886-3p on the CDC6 3′ UTR along with the mutations in the predicted seed region. C) Left figure shows luciferase activity of pEZX-WT-UTR in TPC-1 cells when co-transfected with pre-miR-886-3p or pre-miR-NC, p<0.05. Middle and right figures showluciferase activity of pEZX-Mut-UTR-01 and pEZX-Mut-UTR-02 in TPC-1 cells when co-transfected with pre-miR-886-3p or pre-miR-NC, respectively. All luciferase measurements were made in triplicates and readings were performed at 24 hours post-transfection.