Analysis of PCR products using agarose gel electrophoresis.
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posted on 2012-11-13, 01:24 authored by Karthick Raja Muthu Raja, Lenka Kubiczkova, Lucie Rihova, Martin Piskacek, Pavla Vsianska, Renata Hezova, Ludek Pour, Roman HajekAfter RT-PCR, FoxP3 and GAPDH PCR products were analyzed using agarose gel (2%) electrophoresis. Three HD and three MM patients PCR products from CD4 Treg cells and CD8 Treg cells are shown. Also, FoxP3 and GAPDH PCR products from non-regulatory T cells of a MM patient and a healthy donor are presented. PCR products, 61 bp and 118 bp represent FoxP3 and GAPDH, respectively. Treg cells, T regulatory cells; Non-Treg cells; non-regulatory T cells; MM, multiple myeloma; HD, healthy donor; FoxP3, forkhead-winged helix transcription factor; GAPDH, human glyceraldehyde 3-phosphate dehydrogenase.
