Analysis of CagI and CagL production, and of cagL transcription, in cagI mutants and complemented strains.

(A) Whole cell lysates of the P12 wild-type strain and the indicated cagI mutants or complemented mutants were examined for CagI and CagL production by immunoblot analysis. Numbers in brackets indicate the plasmids used for mutant generation or complementation. (B) The indicated strains were grown in liquid culture to mid-exponential phase, and harvested for total RNA preparation and cDNA synthesis. Transcript levels of cagL were analyzed by quantitative real-time PCR (qPCR) and normalized to levels of 16S-rRNA for each cDNA sample. Bars represent mean values and standard deviations of cagL transcript levels in relation to wild-type levels for at least three independent RNA preparations.