Analysis and confirmation of a differently expressed novel exon-exon junction identified for the ATAD2 gene.

(A) A schematic diagram of the differently expressed novel junction in ATAD2. Red line represents tumor tissues and blue line represents adjacent non-cancerous tissues. The structure of gene was plotted at the bottom. The connecting lines below exons represent all known annotated junctions. The red connecting line above exons represents the predicted novel exon-exon junction. (B) Relative expression of the novel junction of ATAD2 in HCC and non-cancerous tissues. (C) RT-PCR analysis on agrose gel electrophoresis for the novel junction in HCC and non-cancerous tissues from 2 HCC patients. HPRT1 was used as the internal control. (D) A schematic diagram of amino acid sequence for exon 6–8 in ATAD2 gene.