Agarose gel electrophoresis detection of the overlap PCR product from the BnPRP1 gene and the recombinant plasmids pET30a/His-EDDIE-GFP and pET30a-EDDIE-BnPRP1.
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posted on 2015-09-18, 03:17 authored by Huihui Cao, Tao Ke, Renhu Liu, Jingyin Yu, Caihua Dong, Mingxing Cheng, Junyan Huang, Shengyi Liu(A) Lane M, 100 bp DNA marker; Lanes 1–2, Overlap PCR products from the BnPRP1 gene. (B) Lane M, 1 kb DNA marker; Lane 1, recombinant vector pET30a/His-EDDIE-BnPRP1; Lane 2, vector pET30a/His-EDDIE-GFP. C: Lane M, DL2000 DNA marker; Lane 1, positive control; Lane 2, negative control; Lanes 3 and 4, PCR detection of the BnPRP1 gene.
